Drosophila MFAP1 Is Required for Pre-mRNA Processing and G2/M Progression *

摘要: The mammalian spliceosome has mainly been studied using proteomics. isolation and comparison of different splicing intermediates revealed the dynamic association more than 200 factors with spliceosome, relatively few which have in detail. Here, we report characterization Drosophila homologue microfibril-associated protein 1 (dMFAP1), a previously uncharacterized found some human spliceosomal fractions ( Jurica, M. S., Moore, J. (2003) Mol. Cell 12, 5-14 ). We show that dMFAP1 binds directly to Prp38p (dPrp38), tri-small nuclear ribonucleoprotein component Xie, J., Beickman, K., Otte, E., Rymond, B. C. (1998) EMBO 17, 2938-2946 ), is required for pre-mRNA processing. dMFAP1, like dPrp38, essential viability, our vivo data cells reduced levels or dPrp38 proliferate slowly normal undergo apoptosis. Consistent this, double-stranded RNA-mediated depletion causes arrest G(2)/M, this paralleled by reduction mRNA mitotic phosphatase string/cdc25. Interestingly wide range core elicits similar phenotype, suggesting observed G(2)/M might be general consequence interfering function.