Graded requirement for the spliceosome in cell cycle progression.

摘要: Genome stability is ensured by multiple surveillance mechanisms that monitor the duplication, segregation, and integrity of genome throughout cell cycle. Depletion components spliceosome, a macromolecular machine essential for mRNA maturation gene expression, has been associated with increased DNA damage cycle defects. However, specific role spliceosome in these processes remained elusive, as different defects have reported depending on subunit depleted. Through detailed analysis after depletion, we demonstrate required progression through phases Strikingly, phenotype observed depletion correlates extent depletion. Partial core component results at later stages (G2 mitosis), whereas more complete same elicits an early arrest G1. We propose quantitative model which functional dosages are transitions.