Identification of an activator protein for myosin light chain kinase as the Ca2+-dependent modulator protein.
作者： M. Yazawa , K. Yagi , M. Ohshima , S. Kakiuchi , K. Uenishi
DOI: 10.1016/S0021-9258(17)34871-8
关键词: Immunoglobulin light chain 、 Phosphodiesterase 、 Activator (genetics) 、 Myosin 、 Myosin light-chain kinase 、 Skeletal muscle 、 Biology 、 Phosphorylation 、 Biochemistry 、 Adenylate kinase
摘要: Myosin light chain kinase which phosphorylates g2 of skeletal muscle myosin requires an activator for the activity (Yazawa, M., and Yagi, K (1977) J. Biochem. (Tokyo) 82, 287-289). This has now been identified as modulator protein known to be a Ca2+-dependent regulator phosphodiesterase, adenylate cyclase, ATPases. The identification is based on quantitative cross-reactivity brain in activating phosphodiesterase identical properties both proteins regard sensitivities Ca2+, UV absorption spectra, difference spectra with or without mobilities upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In presence protein, was reversibly controlled by physiological concentration Ca2+. We suggest that two Ca2+-receptive proteins, i.e. troponin-C, may play roles contraction-relaxation cycle muscle.
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