A Macrophage-Based Method for the Assessment of the Reactive Oxygen Species (ROS) Activity of Atmospheric Particulate Matter (PM) and Application to Routine (Daily-24 h) Aerosol Monitoring Studies

摘要: Both short- and long-term exposure to particulate matter (PM) air pollution have been demonstrated cause increases in cardiovascular disease, cancer, respiratory disorders. Although the specific mechanisms by which PM these affects are unclear, significant evidence has accumulated suggest that leads increased inflammation as result of excessive production reactive oxygen species (ROS) critical cell types. In order better understand how real-world causes adverse health effects, there is a need efficiently integrate metrics toxicity into large scale monitoring effects/epidemiology studies. Here we describe rapid, inexpensive, method can be employed assess potential sub-mg masses generate oxidative stress alveolar macrophage cells. Importantly, approach compatible with routine daily sampling programs such those administered EPA (Speciation trends network (STN), IMPROVE netw...