Binding of cellular repressor protein or the IE2 protein to a cis-acting negative regulatory element upstream of a human cytomegalovirus early promoter.
作者： L Huang , M F Stinski
DOI: 10.1128/JVI.69.12.7612-7621.1995
关键词: Immediate early protein 、 Binding site 、 Repressor 、 Negative regulatory element 、 Biology 、 Transcription (biology) 、 Upstream activating sequence 、 TATA box 、 Molecular biology 、 CAAT box
摘要: We have previously shown that the human cytomegalovirus early UL4 promoter has upstream negative and positive cis-acting regulatory elements. In absence of region, element confers strong transcriptional activity. The contains a CCAAT box dyad symmetry binds cellular transcription factor NF-Y. effect is negated by viral IE2 protein (L. Huang, C.L. Malone, M.F. Stinski, J. Virol. 68:2108, 1994). investigated binding or to region. major was located between -168 -134 bp relative start site. This could be transferred heterologous promoter, it functioned in either orientation. Mutational analysis demonstrated core DNA sequence element, 5'-GTTTGGAATCGTT-3', required for repressor protein(s) protein. activity present both nonpermissive HeLa permissive fibroblast cells but more abundant cells. Binding correlates with repression from promoter. negation repressive effect.
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