Human Cytomegalovirus Immediate-Early Gene 2 Expression Leads to JCV Replication in Nonpermissive Cells via Transcriptional Activation of JCV T Antigen

摘要: Human papovavirus JCV is the causative agent of demyelinating brain disease progressive multifocal leukoencephalopathy (PML) that typically develops as a complication impaired immunocompetence. displays strong tropism for glial cells which correlated by glial-specific transcriptional regulation viral gene expression. In previous report HCMV was shown to overcome restricted cell specificity inducing DNA replication PML-derived strain in human fibroblasts are nonpermissive alone. Here we show productive induced glioblastoma cells. Both and cells, immediate-early transactivator 2 (IE2) sufficient mediate replication. Furthermore, IE2 induces several structurally different brain- or kidney-derived variants. IE2-induced accompanied induction T antigen expression due stimulation early promoter. Our results indicate HCMV-IE2 JCV.