Presence of T3SS2β genes in trh⁺ Vibrio parahaemolyticus isolated from seafood harvested along Mangalore coast, India.

作者: BK Kumar , VK Deekshit , P Rai , M Shekar , I Karunasagar

DOI: 10.1111/LAM.12210

关键词: PathogenVirulenceGeneHemolysinVibrio parahaemolyticusMicrobiologyBiologyHealth riskPathogenicity

摘要: UNLABELLED Vibrio parahaemolyticus is a seafood-borne pathogen autochthonous to the marine and estuarine ecosystem, responsible for gastroenteritis when contaminated raw seafood consumed. The pathogenicity has been associated with thermostable direct haemolysin (TDH) TDH-related (TRH). Of late, presence of T3SS2α T3SS2β gene clusters well documented in clinical isolates known play an essential role pathogenesis. However, reports on T3SSβ genes V. isolated from and/or environmental samples are scanty. In this study, we have identified analysed distribution harvested along southwest coast India. Results showed that solely trh⁺ tdh⁺ /trh⁺ strains parahaemolyticus. Reverse transcriptase PCR (RT-PCR) were transcriptionally active. To our knowledge, study appears be first description T3SS2β-positive T3SS2 other virulence factors will help better understanding risk illness due SIGNIFICANCE AND IMPACT OF THE STUDY T3SSs (α or β) important contribute their humans. This demonstrated Mangalore coast. RT-PCR found functional. best such as TDH TRH highlights potential health consumers.

参考文章(36)
N.F. Noriea III, C.N. Johnson, K.J. Griffitt, D.J. Grimes, Distribution of type III secretion systems in Vibrio parahaemolyticus from the northern Gulf of Mexico Journal of Applied Microbiology. ,vol. 109, pp. 953- 962 ,(2010) , 10.1111/J.1365-2672.2010.04722.X
Sohini Mukherjee, Gladys Keitany, Yan Li, Yong Wang, Haydn L Ball, Elizabeth J Goldsmith, Kim Orth, Yersinia YopJ Acetylates and Inhibits Kinase Activation by Blocking Phosphorylation Science. ,vol. 312, pp. 1211- 1214 ,(2006) , 10.1126/SCIENCE.1126867
Kim Orth, Lance E Palmer, Zhao Qin Bao, Scott Stewart, Amy E Rudolph, James B Bliska, Jack E Dixon, Inhibition of the Mitogen-Activated Protein Kinase Kinase Superfamily by a Yersinia Effector Science. ,vol. 285, pp. 1920- 1923 ,(1999) , 10.1126/SCIENCE.285.5435.1920
Rabindra N. Bhattacharjee, Kwon-Sam Park, Kazuhisa Okada, Yutaro Kumagai, Satoshi Uematsu, Osamu Takeuchi, Shizuo Akira, Tetsuya Iida, Takeshi Honda, Microarray analysis identifies apoptosis regulatory gene expression in HCT116 cells infected with thermostable direct hemolysin-deletion mutant of Vibrio parahaemolyticus Biochemical and Biophysical Research Communications. ,vol. 335, pp. 328- 334 ,(2005) , 10.1016/J.BBRC.2005.07.080
Lingling Zhang, Kim Orth, Virulence determinants for Vibrio parahaemolyticus infection. Current Opinion in Microbiology. ,vol. 16, pp. 70- 77 ,(2013) , 10.1016/J.MIB.2013.02.002
P RAGHUNATH, S ACHARYA, A BHANUMATHI, I KARUNASAGAR, I KARUNASAGAR, Detection and molecular characterization of Vibrio parahaemolyticus isolated from seafood harvested along the southwest coast of India Food Microbiology. ,vol. 25, pp. 824- 830 ,(2008) , 10.1016/J.FM.2008.04.002
Jennifer E. Trosky, Sohini Mukherjee, Dara L. Burdette, Margaret Roberts, Linda McCarter, Richard M. Siegel, Kim Orth, Inhibition of MAPK Signaling Pathways by VopA from Vibrio parahaemolyticus Journal of Biological Chemistry. ,vol. 279, pp. 51953- 51957 ,(2004) , 10.1074/JBC.M407001200
Shun-Ichi Honda, Ikuo Goto, Ichirou Minematsu, Nagashige Ikeda, Nobuo Asano, Masanori Ishibashi, Yoshio Kinoshita, Mitsuaki Nishibuchi, Takeshi Honda, Toshio Miwatani, Gastroenteritis due to Kanagawa negative Vibrio parahaemolyticus. The Lancet. ,vol. 329, pp. 331- 332 ,(1987) , 10.1016/S0140-6736(87)92062-9
Guy R. Cornelis, The type III secretion injectisome Nature Reviews Microbiology. ,vol. 4, pp. 811- 825 ,(2006) , 10.1038/NRMICRO1526
Christopher A. Broberg, Thomas J. Calder, Kim Orth, Vibrio parahaemolyticus cell biology and pathogenicity determinants. Microbes and Infection. ,vol. 13, pp. 992- 1001 ,(2011) , 10.1016/J.MICINF.2011.06.013