Determining the effects of lipophilic drugs on membrane structure by solid-state NMR spectroscopy: the case of the antioxidant curcumin.

摘要: Curcumin is the active ingredient of turmeric powder, a natural spice used for generations in traditional medicines. Curcumin’s broad spectrum antioxidant, anticarcinogenic, antimutagenic, and anti-inflammatory properties makes it particularly interesting development pharmaceutical compounds. Because curcumin’s various effects on function numerous unrelated membrane proteins, has been suggested that affects bilayer itself. However, detailed atomic-level study interaction curcumin with membranes not attempted. A combination solid-state NMR differential scanning calorimetry experiments shows strong effect structure at low concentrations. inserts deep into transbilayer orientation, anchored by hydrogen bonding to phosphate group lipids manner analogous cholesterol. Like cholesterol, induces segmental ordering membrane. Analysis concentrati...