Multiphoton Microscopy Advances Toward Super Resolution

摘要: Multiphoton microscopy has emerged as a key technique for variety of applications in the life sciences. Its distinct optical sectioning property, due to its nonlinear dependence on illumination intensity, makes it method choice vivo and vitro imaging deep-tissue structures live animal imaging. This further improves visualization complex dynamic biological processes with minimal invasion photodamage. integrated superresolution techniques such 4pi, PALM, STED opened up various possibilities including nanoscale In this chapter we review fundamental multiphoton excitation process advances made exciting field within biophysics framework. is will continue have remarkable times come, undoubtedly fostering new applications.