The uronic acids assay: a method for the determination of chemical activity on biofilm EPS.
作者： Kristina DA Mojica , Michael J Cooney , None
DOI: 10.1080/08927010903503334
关键词: Biology 、 Biochemistry 、 Biofilm 、 Bioassay 、 Bacterial growth 、 Polysaccharide 、 Sodium dodecyl sulfate 、 Uronic acid 、 Chromatography 、 Biofouling 、 Sodium hypochlorite
摘要: In this work, the uronic acids assay was evaluated for its potential to function as a bioassay screen antagonistic activity against production of microbial biofilm exopolysaccharide (EPS). The first applied biofilms produced in presence two universal disinfectants (sodium hypochlorite and sodium dodecyl sulfate) known inhibit growth formation. performance then characterized through statistical assessment threshold concentrations disinfection efficiency consistency relative values reported literature. utility screening enzymatic or chemical inhibitors formation (eg glycosidases, halogenated furanones, semi-crude fractions extracted from minimally fouled marine plants) ability distinguish between true anti-biofilm simple disinfection. Activity (i) no effect, (ii) positive effect (ie increased E...
cabdirect.org
informaworld.com参考文章(40)
Christopher L. Wiatr, Enzyme blend containing cellulase to control industrial slime ,(1989)
L Gram, R de Nys, R Maximilien, M Givskov, P Steinberg, S Kjelleberg, Inhibitory Effects of Secondary Metabolites from the Red Alga Delisea pulchra on Swarming Motility of Proteus mirabilis. Applied and Environmental Microbiology. ,vol. 62, pp. 4284- 4287 ,(1996) , 10.1128/AEM.62.11.4284-4287.1996
J W Costerton, Z Lewandowski, D DeBeer, D Caldwell, D Korber, G James, Biofilms, the customized microniche. Journal of Bacteriology. ,vol. 176, pp. 2137- 2142 ,(1994) , 10.1128/JB.176.8.2137-2142.1994
Sergey Dobretsov, Hans-Uwe Dahms, Huang YiLi, Martin Wahl, Pei-Yuan Qian, The effect of quorum‐sensing blockers on the formation of marine microbial communities and larval attachment FEMS Microbiology Ecology. ,vol. 60, pp. 177- 188 ,(2007) , 10.1111/J.1574-6941.2007.00285.X
Joe J Harrison, Raymond J Turner, Howard Ceri, High-throughput metal susceptibility testing of microbial biofilms. BMC Microbiology. ,vol. 5, pp. 53- 53 ,(2005) , 10.1186/1471-2180-5-53
Sergey V Dobretsov, Pei-Yuan Qian, Effect of Bacteria Associated with the Green Alga Ulva reticulata on Marine Micro- and Macrofouling Biofouling. ,vol. 18, pp. 217- 228 ,(2002) , 10.1080/08927010290013026
S Huang, MG Hadfield, Composition and density of bacterial biofilms determine larval settlement of the polychaete Hydroides elegans Marine Ecology Progress Series. ,vol. 260, pp. 161- 172 ,(2003) , 10.3354/MEPS260161
Tullia M.C.C. Filisetti-Cozzi, Nicholas C. Carpita, Measurement of uronic acids without interference from neutral sugars Analytical Biochemistry. ,vol. 197, pp. 157- 162 ,(1991) , 10.1016/0003-2697(91)90372-Z
Clarissa J. Nobile, Aaron P. Mitchell, Microbial biofilms: e pluribus unum. Current Biology. ,vol. 17, ,(2007) , 10.1016/J.CUB.2007.02.035
OO Lee, PY Qian, Potential control of bacterial epibiosis on the surface of the sponge Mycale adhaerens Aquatic Microbial Ecology. ,vol. 34, pp. 11- 21 ,(2004) , 10.3354/AME034011