The effect of quorum‐sensing blockers on the formation of marine microbial communities and larval attachment

摘要: We studied the effect of quorum-sensing (QS) blockers 5-hydroxy-3[(1R)-1-hydroxypropyl]-4-methylfuran-2(5H)-one (FUR1), (5R)-3,4-dihydroxy-5-[(1S)-1,2-dihydroxyethyl]furan-2(5H)-one (FUR2) and triclosan (TRI) on formation bacterial biofilms, these biofilms larval attachment polychaete Hydroides elegans bryozoan Bugula neritina. 14-day-old subtidal were harvested from artificial substrata allowed to develop in laboratory with without QS blockers. inhibited production violacein by reporter strain Chromobacterium violaceum CV026 did not affect metabolic activity bacteria multispecies biofilms. At a concentration 10(-3) M all three tested compounds establishment microbial communities, but at one 10(-4) only FUR2 establishment. The caused changes density community structure, as revealed terminal restriction fragment length polymorphism FISH. groups most affected Alphaproteobacteria, Gammaproteobacteria Cytophagales. Larvae H. B. neritina avoided settling that had developed presence Our results suggest directly control multi-species indirectly - means biofilm properties modified