Quantification and partial characterisation of regulatory peptides in the liver fluke, Fasciola hepatica, from different mammalian hosts.
作者： R.M. Magee , I. Fairweather , C. Shaw , J.M. McKillop , W.I. Montgomery
DOI: 10.1016/0742-8413(91)90100-8
关键词: Antiserum 、 Gastrin-releasing peptide 、 Peptide 、 Pancreatic polypeptide 、 Radioimmunoassay 、 Biochemistry 、 Liver fluke 、 Peptide PHI 、 Fasciola hepatica 、 Biology
摘要: Abstract 1. Extracts of the liver fluke, Fasciola hepatica from three different hosts (cow, sheep, rat) have been subjected to radioimmunoassay using antisera 6 mammalian regulatory peptides. 2. Immunoreactivity was measured pancreatic polypeptide, substance P, peptide histidine isoleucine and gastrin-releasing peptide. Levels each varied considerably in flukes hosts. 3. Reverse-phase HPLC rat sheep fluke extracts revealed molecular forms tachykinin immunoreactivity single peaks polypeptide immunoreactivity. No GRP-immunoreactivity detected by RIA fractions.
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