Mechanism of oligonucleotide uptake by cells: involvement of specific receptors?
作者： L A Yakubov , E A Deeva , V F Zarytova , E M Ivanova , A S Ryte
关键词: Oligonucleotide 、 Oligomer 、 Mechanism of action 、 RNA 、 Cell 、 Biochemistry 、 Receptor 、 Biology 、 Endocytosis 、 Cell culture
摘要: We have investigated the interaction of oligonucleotides and their alkylating derivatives with mammalian cells. In experiments L929 mouse fibroblast Krebs 2 ascites carcinoma cells, it was found that cellular uptake oligodeoxynucleotide is achieved by an endocytosis mechanism. Uptake considerably more efficient at low oligomer concentration (less than 1 microM), because this a significant percentage total pool absorbed on cell surface internalized absorptive process. Two modified proteins were detected in fibroblasts treated oligonucleotide derivatives. The binding oligomers to inhibited other oligodeoxynucleotides, single- double-stranded DNA, RNA. polyanions heparin chondroitin sulfates A B do not inhibit binding. These observations suggest involvement specific receptor
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