An improved cyan fluorescent protein variant useful for FRET.
作者： Mark A Rizzo , Gerald H Springer , Butch Granada , David W Piston
DOI: 10.1038/NBT945
关键词: Quantum yield 、 Biochemistry 、 Biosensor 、 Fluorophore 、 Yellow fluorescent protein 、 Biophysics 、 Fluorescence 、 Cerulean 、 Site-directed mutagenesis 、 Biology 、 Förster resonance energy transfer
摘要: … proteins derived from Aequorea victoria, ECFP 1 and YFP 2 , have appropriate fluorescence … the major disadvantages of using ECFP in FRET experiments is ECFP's dim fluorescence, …
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