Flybow to dissect circuit assembly in the Drosophila brain.
作者： Nana Shimosako , Dafni Hadjieconomou , Iris Salecker
DOI: 10.1007/978-1-62703-655-9_4
关键词:
摘要: Visualization of single neurons within their complex environment is a pivotal step towards uncovering the mechanisms that control neural circuit development and function. This chapter provides detailed technical information on how to use Drosophila variants mouse Brainbow-2 system, called Flybow, for stochastic labeling cells with different fluorescent proteins in one sample. We first describe genetic strategies heat shock regime required induction recombination events. followed by protocol as prepare samples imaging. Finally, we provide specifications facilitate multichannel image acquisition using confocal microscopy.
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sci-hub.st 参考文章(17)
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