Bright cyan fluorescent protein variants identified by fluorescence lifetime screening
作者： Joachim Goedhart , Laura van Weeren , Mark A Hink , Norbert O E Vischer , Kees Jalink
DOI: 10.1038/NMETH.1415
关键词:
摘要: Optimization of autofluorescent proteins by intensity-based screening bacteria does not necessarily identify the brightest variant for eukaryotes. We report a strategy to screen excited state lifetimes, which identified cyan fluorescent with long fluorescence lifetimes (>3.7 ns) and high quantum yields (>0.8). One variant, mTurquoise, was 1.5-fold brighter than mCerulean in mammalian cells decayed mono-exponentially, making it an excellent resonance energy transfer (FRET) donor.
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