Solution mixing and the emission of light in flow-cells for chemiluminescence detection

摘要: Constructing flow-through reactors for chemiluminescence detection by machining channels into polymer disks has enabled the exploration of new configurations and materials that can improve signal intensity beyond attainable with traditional coiled-tubing design. Several approaches to merge reactant solutions were examined: an intersection, chamber or deeper well in centre a serpentine configuration flow-cell (directly front photomultiplier tube), confluence point outside zone. For several analytically useful, rapid reactions, single-inlet external Y-piece was most suitable, but others (such as KMnO4/Mn(II) morphine, [Ir(f-ppy)2BPS]− fluoroquinolones) dual-inlet provided greater signals. The introduction central mixing zones larger widths than channel reduced response. reversing turns promote efficient intensities spiral channel, increasing sharpness created areas poor solution flow decreased Teflon impregnated glass microspheres increased signals 13%–17%, due reflection stray light towards photodetector.