[19] Extraction of water-soluble enzymes and proteins from membranes

摘要: Publisher Summary This chapter discusses the extraction of water-soluble enzymes and proteins from membranes. Two classes have been isolated The first these includes those which are present in space membrane surrounds, but not true components itself. second class intrinsic which, when separated their normal lipid environment, acquire characteristics classical proteins. several chemical physical methods employed for Exposure to sonic vibrations has proved be an effective means disrupting cells subcellular membranes also widely used as aid solubilizing efficiency solubilization by oscillations is influenced power output instrument, duration exposure volume material processed. Mechanical tissue cell homogenizers solubilize Proteins such cytochrome c may associated with protein or phospholipid through electrostatic interactions.