Quantitative characterization of agglomerates and aggregates of pyrogenic and precipitated amorphous silica nanomaterials by transmission electron microscopy.

摘要: The interaction of a nanomaterial (NM) with biological system depends not only on the size its primary particles but also size, shape and surface topology aggregates agglomerates. A method based transmission electron microscopy (TEM), to visualize NM image analysis, measure detected features quantitatively, was assessed for capacity characterize agglomerates precipitated pyrogenic synthetic amorphous silicon dioxide (SAS), or silica, NM. Bright field (BF) TEM combined systematic random imaging semi-automatic analysis allows measuring properties SAS quantitatively. Automation multiple arithmetically complex parameters simultaneously high numbers particles. This reduces operator-induced bias assures statistically relevant number measurements, avoiding tedious repetitive task manual measurements. Access further selecting optimal parameter in function specific purpose. Using principle component (PCA), twenty-three measured were classified into three classes containing measures presented detailed quantitative characterization NM, like dispersions number-based distributions their mean diameter, sphericity factor.