Semi-automatic size measurement of primary particles in aggregated nanomaterials by transmission electron microscopy

摘要: Abstract A combination of transmission electron microscopic imaging and semi-automatic image analysis allows detecting measuring the primary particles aggregated nanomaterials. The median particle size, number based size distribution, percentage nanoparticles volume specific surface area powdered nanomaterials are determined in framework European Commission's nanomaterial definition. principle a semi-automated approach to measure minimal one dimension aggregates by microscopy is developed using model powdered, titanium dioxide representative test NM-100 with mean diameter near 100 nm limit. detected on watershed segmentation their overlap coefficient measured an Euclidean distance map. contain 22% nanoparticles, between 1 nm 100 nm. method evaluated other TiO2, synthetic amorphous silica carbon black for which nearly 100%. high level automation maximal inscribed circle efficiently. shown be commutable Feret min measurements. standard determine fractal properties fractal-like refined supporting measurements each individual particle.