Scaffoldless tissue engineering of stem cell derived cavernous tissue for treatment of erectile function.

摘要: ABSTRACT Introduction As one-third of erectile dysfunction (ED) patients do not respond to phosphodiesterase-5 inhibitors, there is great demand for new therapeutic options. Adipose tissue-derived stem cells (ADSCs) represent an ideal source ED treatment. Aim To test if ADSCs can be differentiated into smooth muscle (SMCs) and endothelial (ECs), these used engineer cavernous tissue, this engineered tissue will remain long time after implantation integrate corporal tissue. Method Rat were isolated SMC ECs. The labeled with 5-ethynyl-2-deoxyuridine (EdU) construct This was implanted in penises normal rats. rats sacrificed 1 2 months; penis bone marrow collected assess cell survival inclusion the penile tissues. Main Outcome Measures phenotype conversion checked using morphology, immunocytochemistry (immunohistochemistry [IHC]), Western blot EC markers. formation assessed rat antibody (RECA), calponin, collagen. incorporation evaluated hematoxylin eosin, Masson's trichrome, IHC (RECA, EdU). Results confirmed positive staining markers blot. formed exhibited architecture comparable ECs extracellular matrix formation. survived significant numbers months. They showed proof differentiation Conclusions results ability differentiate form survive made forms technology improvement vivo Orabi H, Lin G, Ferretti L, C-S, Lue TF. Scaffoldless engineering derived treatment function. J Sex Med 2012;9:1522–1534.