MESENCHYMAL STEM CELL DELIVERY INTO RAT INFARCTED MYOCARDIUM USING A POROUS POLYSACCHARIDE-BASED SCAFFOLD: A QUANTITATIVE COMPARISON WITH ENDOCARDIAL INJECTION
作者： Catherine Le Visage , Olivier Gournay , Najah Benguirat , Sofiane Hamidi , Laeticia Chaussumier
DOI: 10.1089/TEN.TEA.2011.0053
关键词: Endocardium 、 Mesenchymal stem cell 、 Transplantation 、 Fibrosis 、 Scaffold 、 Chemistry 、 Vascular endothelial growth factor 、 Biomedical engineering 、 Viral vector 、 Green fluorescent protein 、 Cell biology
摘要: The use of mesenchymal stem cells (MSCs) for tissue regeneration is often hampered by modest engraftment in host tissue. This study was designed to quantitatively compare MSCs rates after delivery using a polysaccharide-based porous scaffold or endocardial (EC) injection rat myocardial infarction model. Cellular measured quantitative reverse transcription-polymerase chain reaction previously transduced with lentiviral vector that expresses green fluorescent protein (GFP). promoted local cellular and survival. number residual GFP(+) greater the than EC (9.7% vs. 5.1% at 1 month 16.3% 6.1% 2 months, respectively [n=5]). concurred significant increase mRNA vascular endothelial growth factor level group (p<0.05). Clusters GFP+ were detected peri-infarct area, mainly phenotypically consistent immature MSCs. Functional assessment echocardiography months postinfarct also showed trend toward lower left ventricular dilatation reduced fibrosis comparison direct (n=10). These findings demonstrate biodegradable promising method improve cell into damaged myocardium.
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