Fucoidan in a 3D scaffold interacts with vascular endothelial growth factor and promotes neovascularization in mice

摘要: The aim of this study was to functionalize 3D porous cross-linked scaffolds with natural non-animal sulfated polysaccharide fucoidans in order allow a delivery vascular endothelial growth factor (VEGF) and potentiate its angiogenic activity. Microporous (20 μm) macroporous (200 μm) were functionalized low, medium, or high molecular weight (named LMWF, MMWF, HMWF, respectively). In vitro, addition promoted progenitor cells proliferation both micro- scaffolds. While control without loaded VEGF165 (100 ng) showed fast burst release PBS during the first 24 h, MMWF significantly reduced (p < 0.001). Surface plasmon resonance experiments confirmed direct interaction between VEGF165, characterized by an affinity K D (K d/K a) 1 × 10−9 M. subcutaneous angiogenesis model mice, fucoidan more intense vascularization response than groups. Expression isolectin-B4 α-smooth muscle actin, as well confinement erythrocytes, demonstrated neoformed blood vessels functionality. There significant difference neovessel area density MMWF+VEGF165 (p < 0.001 for all cases). Here, we demonstrate that sequesters delivers biological cues promoting angiogenesis. conclusion, shows hydrogels can formation mature vasculature through local be useful tool ischemic tissues guide therapeutic