Functional divergence between closely related Baeyer-Villiger monooxygenases from Aspergillus flavus

摘要: Baeyer-Villiger monooxygenases (BVMOs) catalyse the chemo-, regio- and enantioselective oxidation of ketones to esters lactones. To date, most cloned BVMOs available are derived from bacteria, although oxidations using fungi have frequently been demonstrated. Here we report cloning characterization four fungus Aspergillus flavus NRRL3357. Phylogenetic analysis shows these cluster in a distinct group apart other well-characterized including cyclohexanone, phenylacetone 4-hydroxyacetophenone monooxygenase. Building on Grogan classification/clustering BVMOs, designated this new Group VI. VI show an early divergence cyclopentanone monooxygenase (CPMO) type (Group I). Substrate profiling cyclic, bicyclic, aliphatic aryl clear function specificity not only between CPMO-type but also A. BVMO paralogues despite their high sequence similarity. This study contributes growing number addresses current classification Type I usefulness phylogenetic clustering prediction selectivity when genome-mining is used search for biocatalysts.