Comparison of Different Signal Sequences to Use for Periplasmic Over-Expression of Buforin I in Escherichia coli: An In Silico Study
作者： Sahar Roshanak , Farideh Tabatabaei Yazdi , Fakhri Shahidi , Ali Javadmanesh , Jebrail Movaffagh
DOI: 10.1007/S10989-020-10042-6
关键词: Periplasmic space 、 Exonuclease 、 Escherichia coli 、 Chemistry 、 Signal 、 Start codon 、 Signal peptide 、 Untranslated region 、 Biochemistry 、 In silico
摘要: Computational prediction of signal peptides is one the most important steps in genetic engineering experiments. The periplasmic expression cause reducing inherent destructive behavior Bofurin I against its host and also susceptibility to proteolytic degradation. In order predict best for Buforin E. coli, 103 sequences were retired from peptide databases. Since purpose this study was introduce optimal expression, first, sub-cellular localization site analyzed. Then, n, h, c regions peptide, probability physico-chemical features investigated. Base on results, MalE, hofQ, papK, ugpB, zraP, sfmC introduced as peptides. For increasing half-life mRNA stability exonuclease activity, secondary structures including Shine-Dalgarno, untranslated region ompA, start codon, Based total free energy pilot evaluated conformations, papK seemed more appropriate than rest obtained result can be used design constructs.
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