Rfx2 is required for spermatogenesis in the mouse

摘要: Summary RFX transcription factors are key regulators of ciliogenesis in vertebrates. In Xenopus and zebrafish embryos, knockdown Rfx2 causes defects neural tube closure left–right axis patterning. To determine the essential role gene mammalian development, we generated Rfx2-deficient mice using an embryonic stem cell clone containing a lacZ trap reporter inserted into first intron gene. We found that is expressed ciliated tissues during mouse development including node, floor plate dorsal tube. However, homozygous for mutation did not have or organ situs. The insertion appears to create null allele as mRNA was detected Rfx2gt/gt embryos. Although do obvious phenotype, males infertile because defect spermatid maturation at before round elongating stage. Our results indicate but required spermatogenesis. genesis 53:604–611, 2015. © 2015 Wiley Periodicals, Inc.