Efficient Generation of Germ Line Transmitting Chimeras from C57BL/6N ES Cells by Aggregation with Outbred Host Embryos
作者： Marina Gertsenstein , Lauryl M. J. Nutter , Tammy Reid , Monica Pereira , William L. Stanford
DOI: 10.1371/JOURNAL.PONE.0011260
关键词:
摘要: Genetically modified mouse strains derived from embryonic stem (ES) cells have become essential tools for functional genomics and biomedical research. Large scale mutagenesis projects are producing libraries of mutant C57BL/6 (B6) ES to enable the annotation every gene genome. To realize utility these resources, efficient accessible methods generating mice necessary. Here, we describe a combination ICR morula aggregation chemically-defined culture medium with widely available components high efficiency generation germline transmitting chimeras C57BL/6N cells. Together will ease access broader research community publicly B6 cell resources.
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Janet Rossant, Elizabeth Merentes-Diaz, Elen Gocza, Eszter Ivanyi, Andras Nagy, Developmental Potential of Mouse Embryonic Stem Cells Springer, New York, NY. pp. 157- 165 ,(1993) , 10.1007/978-1-4613-9317-7_12
Gabriela Pacholczyk, Rupali Suhag, Magdalena Mazurek, Suzanne M. Dederscheck, Pandelakis A. Koni, Generation of C57BL/6 knockout mice using C3H × BALB/c blastocysts BioTechniques. ,vol. 44, pp. 413- 416 ,(2008) , 10.2144/000112706
Colin L. Stewart, Production of chimeras between embryonic stem cells and embryos. Methods in Enzymology. ,vol. 225, pp. 823- 856 ,(1993) , 10.1016/0076-6879(93)25053-5
K. Schuster-Gossler, A.W. Lee, C.P. Lerner, H.J. Parker, V.W. Dyer, V.E. Scott, A. Gossler, J.C. Conover, Use of coisogenic host blastocysts for efficient establishment of germline chimeras with C57BL/6J ES cell lines. BioTechniques. ,vol. 31, pp. 1022- 1026 ,(2001) , 10.2144/01315ST01
Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy, Richard Behringer, Manipulating the Mouse Embryo: A Laboratory Manual ,(2002)
Mineko Maeda, Yuzuru Kubohara, Protein Phosphorylation during the Process of Prestalk-to-Stalk Conversion in Dictyostelium discoideum. (Dictyostelium discoideum/8-bromo cyclic AMP/stalk differentiation/protein kinase A/protein kinase inhibitor) Development, Growth and Differentiation. ,vol. 35, pp. 561- 568 ,(1993) , 10.1111/J.1440-169X.1993.00561.X
W. Auerbach, J.H. Dunmore, V. Fairchild-Huntress, Q. Fang, A.B. Auerbach, D. Huszar, A.L. Joyner, Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines BioTechniques. ,vol. 29, pp. 1024- 1032 ,(2000) , 10.2144/00295ST04
Daniel W. McVicar, Robin Winkler-Pickett, Lynn S. Taylor, Andrew Makrigiannis, Michael Bennett, Stephen K. Anderson, John R. Ortaldo, Aberrant DAP12 Signaling in the 129 Strain of Mice: Implications for the Analysis of Gene-Targeted Mice Journal of Immunology. ,vol. 169, pp. 1721- 1728 ,(2002) , 10.4049/JIMMUNOL.169.4.1721
Janet Rossant, András Nagy, Elen Gócza, Elizabeth Merentes Diaz, Eszter Iványi, Merja Markkula, Valerie R. Prideaux, Embryonic stem cells alone are able to support fetal development in the mouse. Development. ,vol. 110, pp. 815- 821 ,(1990) , 10.1242/DEV.110.3.815
Y. Lallemand, P. Brulet, An in situ assessment of the routes and extents of colonisation of the mouse embryo by embryonic stem cells and their descendants. Development. ,vol. 110, pp. 1241- 1248 ,(1990) , 10.1242/DEV.110.4.1241