Production of Mouse Chimeras by Aggregating Pluripotent Stem Cells with Embryos

摘要: Experimental mouse chimeras have served as immensely important research tools for studying many aspects of mammalian development ever since they first were produced over 50 years ago. Chimera studies crucial assays in the era modern genetics that was triggered by advent embryonic stem cells. Lately, are also used proof pluripotency and normality induced pluripotent With this long history mind, it may seem surprising now an ever-increasing role to play. The high-throughput gene targeting projects process producing ES cell lines with a mutation each close 20,000 known protein coding genes. These will all be waiting germline transmission through chimeras. Such large-scale approach calls simplified methods generating transmitting In chapter, we describe currently most cost efficient simple method; aggregation cells diploid or tetraploid embryos. Since large knockout using C57BL/6 background, pay special attention derived from inbred strain.