Manipulating the Mouse Embryo: A Laboratory Manual

摘要: Chapter 1. Developmental Genetics and Embryology of the Mouse: Past, Present, Future 2. Summary Mouse Development 3. Production Transgenic Chimeric Mice: General Issues 4. Recovery In Vitro Culture Pre-implantation-Stage Embryos 5. Isolation, Culture, Manipulation Post-implantation 6. Surgical Procedures 7. Mice 8. Blastocyst-derived Stem Cells 9. Vector Designs for Embryonic Cell-based Transgenesis Genome Alterations 10. Introduction Foreign DNA into 11. Chimeras 12. Detection Analysis Specific Sequences 13. Parthenogenesis, Pronuclear Transfer, Cloning 14. Assisted Reproduction: Ovary Transplantation, Fertilization, Artificial Insemination, Intracytoplasmic Sperm Injection 15. Cryopreservation, Rederivation, Transport 16. Techniques Visualizing Gene Products, Cells, Tissues, Organ Systems 17. Setting up Equipping a Micromanipulation Lab Appendices