Isolation and Maintenance of Trophoblast Stem Cells

摘要: Trophoblast stem (TS) cell lines can be derived from mouse blastocysts and early trophoblast tissue. This chapter describes protocols reagents for the maintenance derivation of TS lines. Reagents should prepared before attempting to derive or culture The include fibroblast growth factor 4 heparin stocks, media, embryonic fibroblast-conditioned medium (EMFI-CM). Culturing is also described in chapter. cells grow well on standard tissue dishes if supplemented with EMFI-CM. similar (ES) However, success rate considerably higher less expertise required recognize pluripotent colonies. Thawing done properly. Transfection accomplished Lipofectamine Plus.