Regenerative Potential and Inflammation-Induced Secretion Profile of Human Adipose-Derived Stromal Vascular Cells Are Influenced by Donor Variability and Prior Breast Cancer Diagnosis.

摘要: Adipose tissue contains a heterogeneous population of stromal vascular fraction (SVF) cells that work synergistically with resident cell types to enhance healing. Ease access and processing paired therapeutic promise make SVF an attractive option for autologous applications in regenerative medicine. However, inherent variability potential from one patient another hinders prognosis determination any person. This study investigated the properties inflammation responses thirteen, medically diverse human donors. Using non-expanded primary lipoaspirate samples, were assessed robustness several parameters integral regeneration, including yield, viability, self-renewal capacity, proliferation, differentiation potential, immunomodulatory cytokine secretion. Each parameter was selected either its role defined here as ability heal tissues through stem repopulation subsequent multipotent differentiation, or wound healing trophic activity. These data then analyzed consistent predictable patterns between across measurements, while also investigating influence donors’ relevant medical histories, particularly if donor remission following breast cancer treatment. Analyses identified positive correlations among expression three cytokines: interleukin (IL)-6, IL-8, monocyte chemoattractant protein (MCP)-1. The these cytokines positively related capacity. results are potentially establishing expectations both preclinical experiments targeted clinical treatment strategies use patients histories.