A Comparison of Two Separation Modes: HILIC and Aqueous Normal Phase Chromatography

摘要: In the literature (1) and in commercial advertising, one can often find material descriptions of chromatographic retention that use terms hydrophilic interaction liquid chromatography (HILIC) aqueous normal phase (ANP) such a manner they be construed to identical. However, reality, there should (and is) real difference between these two modes types columns achieve them. A more precise definition ANP HILIC is proposed here will enable chromatographers distinguish mechanisms select suitable for particular applications.