Simultaneous gas chromatography-tandem mass spectrometry quantification of symmetric and asymmetric dimethylarginine in human urine
作者： Dimitrios Tsikas , Bibiana Beckmann , Frank-Mathias Gutzki , Jens Jordan
关键词: Spot urine 、 Gas Chromatography/Tandem Mass Spectrometry 、 Excretion 、 Mass spectrometry 、 Symmetric dimethylarginine 、 Chromatography 、 Asymmetric dimethylarginine 、 Quantitative determination 、 Urine 、 Chemistry
摘要: Previously, we demonstrated the utility of a gas chromatography-tandem mass spectrometry (GC-MS/MS) method for quantitative determination asymmetric dimethylarginine (ADMA) in biological samples. Here report extension this to symmetric (SDMA) human urine. SDMA and ADMA were simultaneously quantitated urine by using their situ prepared trideuteromethyl esters as internal standards. The GC-MS/MS was validated spot samples 19 healthy adults. In these samples, creatinine-corrected excretion rate 3.23±0.63 μmol/mmol 3.14±0.98 ADMA.
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