Effects of 1,25(OH)2D3 in experimental mesangial proliferative nephritis in rats

摘要: Effects of 1,25(OH) 2 D 3 in experimental mesangial proliferative nephritis rats. Background 1,25-Dihydroxyvitamin [1,25(OH) ], the active form vitamin , is a potent immunomodulatory agent on several cell types such as monocytes and cells. Recruitment inflammatory cells, well stimulation resident cells matrix accumulation are key features various human glomerular diseases. Here we show that attenuates morphologic functional alterations anti-Thy-1.1. nephritis, an model glomerulonephritis. Methods The anti-Thy-1.1 group (group I) comprised 24 rats at day 0 received 0.5mL saline containing 400 μg monoclonal antibodies (mAb) IgG. treated with II) were given dose 25 ng/100g body wt/day, from -3 to 14. At 0, control III) 12 injected vehicle alone; IV)—12 II without mAb administration. Proteinuria urinary interleukin-6 excretion measured daily. Blood urea nitrogen creatinine, creatinine clearance, calcium, phosphate days 4, 7, In addition conventional periodic acid-Schiff staining, binding IgG C3b complement fraction, His48- ED1–positive studied by immunofluorescence. Mesangial proliferation was proliferating nuclear antigen (PCNA) technique. Apoptosis evaluated TUNEL assay. Results versus alone showed significant reduction protein (at 424 ± 228 vs. 66 30 mg/mg P = 0.02) 3, 537 360 pg/mL 110 34 pg/mg 0.015), reduced diameters 283 38 261 48 μm, Conclusions can reduce hypercellularity, infiltration preserving apoptotic response reparative phase.