Runx2 and Osterix Gene Expression in Human B one Marrow Stromal Cells Are Mediated by Far2Infrared Radiation

摘要: A bstract—Runx2 and Osterix are the key transcription factors initiating regulating early osteogenesis late mineralization of bone. To investigate whether expression these osteogenic genes in human bone marrow stromal cells (hBMSCs) was regulated by physical stimulation, far infrared (FIR) applied to this present work. hBMSCs were cocultured physically separated with their adjacent cell, osteoblasts (hOBs) a transwell system. Both placed onto 200-mg FIR ceramic powders packed into plastic bag for uniform irradiation. 0-, 30-, 60-, 120-min treatment irradiation performed inside an incubator. Each group fully wrapped foils prevent interruption. The electrophoretic RT-PCR data showed that exhibited higher gene Runx2, collagen type I under 120-min/day at day 14. Histochemical staining results also revealed FIR-treated expressed more ALP calcium deposits after 28-day exposure. It suggested appropriate stimulus may be alternative tool control differentiation vitro.