An improved apotryptophanase assay for pyridoxal phosphate.
作者： Betty E. Haskell , Esmond E. Snell
DOI: 10.1016/0003-2697(72)90218-7
关键词: Enzyme 、 Chemistry 、 Tissue extracts 、 Biochemistry 、 Pyridoxal phosphate
摘要: An improved procedure for the estimation of pyridoxal phosphate by apotryptophanase method is described. The depends upon use a highly active preparation from E. coliB1t7A. It colorimetric that permits quantitative measurement nanogram quantities without radioactive substrates. suitable both assaying enzymes whose product and determination in tissue extracts.
elsevier.com
sci-hub.se 参考文章(11)
W. Austin Newton, Y. Morino, Esmond E. Snell, Properties of Crystalline Tryptophanase Journal of Biological Chemistry. ,vol. 240, pp. 1211- 1218 ,(1965) , 10.1016/S0021-9258(18)97562-9
Hiroshi Wada, Esmond E. Snell, The enzymatic oxidation of pyridoxine and pyridoxamine phosphates. Journal of Biological Chemistry. ,vol. 236, pp. 2089- 2095 ,(1961) , 10.1016/S0021-9258(18)64134-1
HM HENDERSON, THE CONVERSION OF PYRIDOXINE PHOSPHATE INTO PYRIDOXAL PHOSPHATE IN ESCHERICHIA COLI. Biochemical Journal. ,vol. 95, pp. 775- 779 ,(1965) , 10.1042/BJ0950775
Salman Gailani, Pyridoxal phosphate determination in isolated leucocytes and tissue by E. coli apotryptophanase. Analytical Biochemistry. ,vol. 13, pp. 19- 27 ,(1965) , 10.1016/0003-2697(65)90114-4
Vincenzo Bonavita, The reaction of pyridoxal 5-phosphate with cyanide and its analytical use. Archives of Biochemistry and Biophysics. ,vol. 88, pp. 366- 372 ,(1960) , 10.1016/0003-9861(60)90252-6
Roseann S. White, Walter B. Dempsey, Purification and properties of vitamin B6 kinase from Escherichia coli B. Biochemistry. ,vol. 9, pp. 4057- 4064 ,(1970) , 10.1021/BI00823A005
Elijah Adams, Fluorometric determination of pyridoxal phosphate in enzymes. Analytical Biochemistry. ,vol. 31, pp. 118- 122 ,(1969) , 10.1016/0003-2697(69)90248-6
Arne Hamfelt, A method of determining pyridoxal phosphate in blood by decarboxylation of L-tyrosine-14C (U). Clinica Chimica Acta. ,vol. 7, pp. 746- 748 ,(1962) , 10.1016/0009-8981(62)90167-5
G. H. Sloane-Stanley, The use of the tyrosine apodecarboxylase of Streptococcus faecalis R for the estimation of codecarboxylase Biochemical Journal. ,vol. 44, pp. 567- 573 ,(1949) , 10.1042/BJ0440567
N O Kaplan, S P Colowick, Jagannathan, M D Joshi, Methods in Enzymology , Vol University Of Nairobi. ,(1966)