Simple Assay for Femtomoles of Pyridoxal and Pyridoxamine Phosphates

摘要: In recent years, there has been a renewed interest in vitamin B-6, sparked part by the discoveries that several population groups are only marginally sufficient vitamin. These include alcoholics (1), pregnant women (2,3), females on oral contraceptive agents (3,4), and senior citizens (1,5-7). Of various methods devised to indicate nutritional status of measurement plasma levels pyridoxal 5’-P is being employed with increasing frequency. Pyridoxal concentration human blood correlates urinary excretion xanthurenic acid (8), enzyme activity coenzyme saturation erythrocyte aspartate aminotransferase (9). rats, bears close relation pyridoxine intake, growth, aspartate, alanine activities muscle store vitamer (10). Assay best carried out enzymatic means because they offer high specificity sensitivity. Currently available protocols involve use apoenzyme tyrosine decarboxylase (11-13), tryptophanase (14,15), wheat germ yeast (6,16), D-serine dehydratase (17), phosphorylase b (18), y-cyanoaminobutyric synthetase (19).