Clioquinol induces pro-death autophagy in leukemia and myeloma cells by disrupting the mTOR signaling pathway

摘要: Clioquinol is an anti-microbial drug, and it was recently found to induce cancer cell death. In the present study, clioquinol trigger autophagy by inducing LC3 lipidation autophagosome formation which abolished inhibitor 3-methyladenine. Further study showed displayed no effects on PI3KC3 or Beclin 1 expression but downregulated enzymatic activity of mammalian target Rapamycin (mTOR), a critical modulator autophagy. Moreover, inhibited catalytic mTOR complex 1, thus suppressing phosphorylation P70S6K 4E-BP1, two major proteins associated with in mTORC1 signaling pathway. induced leukemia myeloma apoptosis, however, addition 3-methyladenine attenuated this kind Therefore, demonstrated that induces apoptosis cells disrupting