Molecular cloning of the ViaB region of Salmonella typhi.
作者： Takayuki Ezaki , Yasuhiro Hashimoto , Na Li , Hiroyuki Yamamoto
DOI: 10.1016/0378-1097(91)90645-Q
关键词: Microbiology 、 Escherichia coli 、 DNA 、 Molecular cloning 、 Salmonella typhi 、 Southern blot 、 Plasmid 、 Biology 、 Recombinant DNA 、 Molecular biology 、 Citrobacter freundii
摘要: The ViaB region required for Vi antigen production in Salmonella typhi was cloned. plasmid pGBM124 containing a 14-kb S. thypi chromosomal DNA fragment conferred the ability to produce on Escherichia coli HB101 and ViaB-deleted GIFU10007-3. Tn5 insertion analysis showed that split into three regions. Region 1 2 are involved biosynthesis of polysaccharide. 3 is translocation polysaccharide cell surface. Southern blot hybridization regions but not 1, were considerably homologous Vi-positive Citrobacter freundii.
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