Microfluidic bead-based methods for DNA analysis
作者： Aman Russom
DOI:
关键词: Melting curve analysis 、 Chip 、 Microheater 、 Materials science 、 Microcontact printing 、 Biomedical engineering 、 SNP genotyping 、 Nanotechnology 、 Genotyping 、 Microfluidics 、 SNP array
摘要: This report describes a rapid solid-phase melting curve analysis method for single-nucleotide polymorphism (SNP) genotyping. The is based on dynamic allele-specific hybridization (DASH). DNA duplexes are conjugated beads that immobilized the surface of microheater chip with integrated heaters and temperature sensors. SNP PCR products were scored, illustrating sensitivity robustness system. random bead immobilization by microcontact printing. Single-bead detection multiplexing performed heating rate more than 20 times faster conventional DASH. Analyses took 15 min could be in less 1 min, enabling ultrarapid analysis. In addition, an array version was implemented preparation arrays high-throughput
参考文章(118)
K. Mullis, F. Faloona, S. Scharf, R. Saiki, G. Horn, H. Erlich, Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction Cold Spring Harbor Symposia on Quantitative Biology. ,vol. 51, pp. 263- 273 ,(1986) , 10.1101/SQB.1986.051.01.032
Mark A Burns, Brian N Johnson, Sundaresh N Brahmasandra, Kalyan Handique, James R Webster, Madhavi Krishnan, Timothy S Sammarco, Piu M Man, Darren Jones, Dylan Heldsinger, Carlos H Mastrangelo, David T Burke, An Integrated Nanoliter DNA Analysis Device Science. ,vol. 282, pp. 484- 487 ,(1998) , 10.1126/SCIENCE.282.5388.484
S. Fodor, J. Read, M. Pirrung, L Stryer, A. Lu, D Solas, Light-directed, spatially addressable parallel chemical synthesis. Science. ,vol. 251, pp. 767- 773 ,(1991) , 10.1126/SCIENCE.1990438
D Shalon, S J Smith, P O Brown, A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization. Genome Research. ,vol. 6, pp. 639- 645 ,(1996) , 10.1101/GR.6.7.639
John M. Shumaker, Andres Metspalu, C. Thomas Caskey, Mutation detection by solid phase primer extension. Human Mutation. ,vol. 7, pp. 346- 354 ,(1996) , 10.1002/(SICI)1098-1004(1996)7:4<346::AID-HUMU9>3.0.CO;2-6
Robi D Mitra, George M Church, In situ localized amplification and contact replication of many individual DNA molecules Nucleic Acids Research. ,vol. 27, pp. 34- ,(1999) , 10.1093/NAR/27.24.E34
US DOE Joint Genome Institute: Hawkins Trevor 4 Branscomb Elbert 4 Predki Paul 4 Richardson Paul 4 Wenning Sarah 4 Slezak Tom 4 Doggett Norman 4 Cheng Jan-Fang 4 Olsen Anne 4 Lucas Susan 4 Elkin Christopher 4 Uberbacher Edward 4 Frazier Marvin 4, RIKEN Genomic Sciences Center: Sakaki Yoshiyuki 9 Fujiyama Asao 9 Hattori Masahira 9 Yada Tetsushi 9 Toyoda Atsushi 9 Itoh Takehiko 9 Kawagoe Chiharu 9 Watanabe Hidemi 9 Totoki Yasushi 9 Taylor Todd 9, Genoscope and CNRS UMR-8030: Weissenbach Jean 10 Heilig Roland 10 Saurin William 10 Artiguenave Francois 10 Brottier Philippe 10 Bruls Thomas 10 Pelletier Eric 10 Robert Catherine 10 Wincker Patrick 10, Department of Genome Analysis, Institute of Molecular Biotechnology: Rosenthal André 12 Platzer Matthias 12 Nyakatura Gerald 12 Taudien Stefan 12 Rump Andreas 12, GTC Sequencing Center: Smith Douglas R. 11 Doucette-Stamm Lynn 11 Rubenfield Marc 11 Weinstock Keith 11 Lee Hong Mei 11 Dubois JoAnn 11, Beijing Genomics Institute/Human Genome Center: Yang Huanming 13 Yu Jun 13 Wang Jian 13 Huang Guyang 14 Gu Jun 15, Multimegabase Sequencing Center, The Institute for Systems Biology: Hood Leroy 16 Rowen Lee 16 Madan Anup 16 Qin Shizen 16, Stanford Genome Technology Center: Davis Ronald W. 17 Federspiel Nancy A. 17 Abola A. Pia 17 Proctor Michael J. 17, University of Oklahoma's Advanced Center for Genome Technology: Roe Bruce A. 22 Chen Feng 22 Pan Huaqin 22, Max Planck Institute for Molecular Genetics: Ramser Juliane 23 Lehrach Hans 23 Reinhardt Richard 23, Cold Spring Harbor Laboratory, Lita Annenberg Hazen Genome Center: McCombie W. Richard 24 de la Bastide Melissa 24 Dedhia Neilay 24, GBF—German Research Centre for Biotechnology: Blöcker Helmut 25 Hornischer Klaus 25 Nordsiek Gabriele 25, Scientific management: National Human Genome Research Institute, US National Institutes of Health: Collins Francis fc23a@ nih. gov 46 b Guyer Mark S. 46 Peterson Jane 46 Felsenfeld Adam 46 Wetterstrand Kris A. 46, Stanford Human Genome Center: Myers Richard M. 18 Schmutz Jeremy 18 Dickson Mark 18 Grimwood Jane 18 Cox David R. 18, University of Washington Genome Center: Olson Maynard V. 19 Kaul Rajinder 19 Raymond Christopher 19, Department of Molecular Biology, Keio University School of Medicine: Shimizu Nobuyoshi 20 Kawasaki Kazuhiko 20 Minoshima Shinsei 20, University of Texas Southwestern Medical Center at Dallas: Evans Glen A. 21 49 50 49 Athanasiou Maria 21 Schultz Roger 21, Office of Science, US Department of Energy: Patrinos Aristides 47, Wellcome Trust: Morgan Michael J. 48, None, Initial sequencing and analysis of the human genome. Nature. ,vol. 409, pp. 860- 921 ,(2001) , 10.1038/35057062
A. Ahmadian, J. Lundeberg, P. Nyrén, M. Uhlén, M. Ronaghi, Analysis of the p53 tumor suppressor gene by pyrosequencing. BioTechniques. ,vol. 28, pp. 140- 147 ,(2000) , 10.2144/00281RR02
Mostafa Ronaghi, Pyrosequencing for SNP genotyping. Methods of Molecular Biology. ,vol. 212, pp. 189- 195 ,(2003) , 10.1385/1-59259-327-5:189
D.R. Greaves, R.K. Patient, AT)n is an interspersed repeat in the Xenopus genome. The EMBO Journal. ,vol. 4, pp. 2617- 2626 ,(1985) , 10.1002/J.1460-2075.1985.TB03979.X