Analysis of the p53 tumor suppressor gene by pyrosequencing.
作者： A. Ahmadian , J. Lundeberg , P. Nyrén , M. Uhlén , M. Ronaghi
DOI: 10.2144/00281RR02
关键词:
摘要: Tumor suppressor genes are implicated in cell cycle progression. Inactivation of these predominantly occurs through mutations and/or allelic loss that involves both alleles. With inactivation by multiple a single gene, cloning the amplified gene is necessary to determine whether reside on one or Using pyrosequencing, recently developed approach based sequencing-by-synthesis, we studied genetic variability p53 tumor and could quantify ratio between mutated wild-type fragments. Furthermore, this sequencing technique also allows determination adjacent with no
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