Immunohistochemical estimation of cell cycle entry and phase distribution in astrocytomas: applications in diagnostic neuropathology

摘要: An immunohistochemical method for assessing cell cycle phase distribution in neurosurgical biopsies would enable such data to be incorporated into diagnostic algorithms the estimation of prognosis and response adjuvant chemotherapy glial neoplasms. without requirement flow cytometric analysis. Paraffin-embedded sections intracerebral gliomas (n = 48). consisting diffuse astrocytoma 9), anaplastic 8) glioblastoma 31), were analysed by immunohistochemistry using markers entry, Mcm-2 Ki6 7, putative phase. cyclins Dl (G1-phase), cyclin A (S-phase), B1 (G2-phase) phosphohistone H3 (Mitosis). Double labelling confocal microscopy confirmed that infrequently coexpressed. Cell estimations corroborated There was a significant increase (P < 0.0001), Ki67 0.0001) Bl 0.002) expression with increasing grade from through glioblastoma, suggesting any these four has potential as marker tumour grade. In subset glioblastomas 16) which accurate clinical follow-up available, there suggestion A:Mcm-2 fraction might predict relatively favourable radical radiotherapy. These provisional findings, however, require confirmation larger study. We conclude it is feasible obtain detailed analysis tissue biopsies. Such information may facilitate grading prognostic value obtained routine laboratory.