Electrospray ionization mass spectrometry of

摘要: Lysoglycerophosphocholine lipids (lyso-GPC) are important intermediates in the synthesis and metabolism of glycerophosphocholine which major components cellular lipid bilayer. Significant differences collisional induced decomposition (CID) behavior were observed for each four different subtypes lyso-GPC both positive negative ions. A difference was initial CID product ions derived from [M + H]+ with loss water that very abundant acyl have a fatty acid ester substituent at either sn- 1 or 2 positions. Loss neutral not prominent case plasmenyl plasmanyl species. The mechanism responsible this consistent higher proton affinity carboxyl carbonyl oxygen atoms vinyl ether found lipids, respectively, as compared to carbinol atom common all Collisional activation − 15]− also revealed distinctive compounds showed facile elimination highly stable carboxylate anion, whereas species underwent fragmentation aldehyde, likely result rearrangement involving double bond moiety. alkyl (plasmanyl lipids) did undergo reaction other permitted differentiation acyl, plasmenyl, subtypes.