Video-enhanced contrast, differential interference contrast (AVEC-DIC) microscopy : a new method capable of analyzing microtubule-related motility in the reticulopodial network of Allogromia laticollaris

摘要: A new method called Allen Video-enhanced Contrast, Differential Interference Contrast (AVEC-DIC) microscopy is shown to be sufficiently sensitive detect several features of microtubule-related motility in the reticulopodial network foraminifer, Allogromia. The takes advantage variable gain and offset a binary video camera operate DIC microscope under conditions highly favorable for imaging, but which optical image virtually invisible eye yet retains its full information when viewed by suitable camera. improvements are made possible setting de Senarmont compensator lambda/9-lambda/4 at maximal working aperture internally corrected planapochromatic objectives. Under these conditions, feature can reject so much stray light from instrument specimen that contrast compares favorably with observed high-extinction images, polarizing rectifiers offer scarcely any advantage. Freed constraints light-limited microscopy, images recorded 60 times per second, or over 1,000 rate photomicrographs comparable magnifications conditions. Application this Allogromia has cytoplasmic organelles translocated only contact single microtubules bundles microtubules, fail move separated microtubules. Microtubules themselves undergo both axial translatory ("sliding") lateral "zipping unzipping" movements have been suggested occur during mitosis other biological processes.