Generation of molecular markers for the identification of isolates of Puccinia jaceae, a potential biological control agent of yellow starthistle

摘要: Abstract Yellow starthistle (Centaurea solstitialis L.) (YST) is an important invasive plant that infests millions of hectares in the western USA and Canada. Isolates exotic rust fungus Puccinia jaceae have been evaluated as a potential biological control for YST. The objectives this study were to characterize isolates P. by amplified fragment length polymorphic (AFLP) DNA analysis develop primers will allow identification monitoring isolates. AFLP indicated Eurasian from different hosts exhibited unique fingerprint patterns primarily based on host/pathogenicity preference. Overall similarities profiles ranged 68.1 94.6%. three originally collected YST clustered with 87.3–94.6%. Simple sequence repeat (SSR) screened fragments pathogens Two primers, (GACA)4 (ACG)5, produced 1170 912-bp fragments, respectively, var. solstitialis. sequenced, designed tested spp. accessions Centaurea Primers GACA 1091-bp product only infect ACG 851-bp same isolates, isolate maculosa, which weak pathogen These be useful may released future field trials manage populations.