Contribution of porcine aminopeptidase N to porcine deltacoronavirus infection.

摘要: Porcine deltacoronavirus (PDCoV), a member of genus Deltacoronavirus, is an emerging swine enteropathogenic coronavirus (CoV). Although outstanding efforts have led to the identification Alphacoronavirus and Betacoronavirus receptors, receptor for Deltacoronavirus unclear. Here, we compared amino acid sequences several representative CoVs. Phylogenetic analysis showed that PDCoV spike (S) protein was close cluster containing transmissible gastroenteritis virus (TGEV), which utilizes porcine aminopeptidase N (pAPN) as functional receptor. Ectopic expression pAPN in non-susceptible BHK-21 cells rendered them susceptible PDCoV. These results indicate may be infection. However, treatment with APN-specific antibody inhibitors did not completely block infection IPI-2I intestinal epithelial cells. knockout blocked TGEV but only slightly decreased Homologous modeling S1 C-terminal domain (S1-CTD) or S1-CTD adopted β-turns (β1-β2 β3-β4), forming tip β-barrel, recognize pAPN. top residues β1-β2 turn had possibility support interaction pAPN, β3-β4 failed contact We also discuss evolution variation based on structure information, providing clues explain usage by Taken together, presented herein reveal likely critical PDCoV, although it involved