Emergence of carbapenem resistant Escherichia coli isolates producing blaNDM and blaOXA-48-like carried on IncA/C and IncL/M plasmids at two Iranian university hospitals
作者： Hamid Solgi , Christian G. Giske , Farzad Badmasti , Shadi Aghamohammad , Seyed Asghar Havaei
DOI: 10.1016/J.MEEGID.2017.10.003
关键词: Plasmid 、 Genetic diversity 、 Replicon 、 Multilocus sequence typing 、 Ertapenem 、 Escherichia coli 、 Biology 、 Pulsed-field gel electrophoresis 、 Microbiology 、 Virology 、 Typing
摘要: The emergence of carbapenem resistance among Escherichia coli is a serious threat to public health. objective this study was investigate genes and clonality resistant E. in Iran. Between February 2015 July 2016, total 32 non-duplicate isolates that were ertapenem or intermediate (R/I-ETP) collected from patient clinical surveillance cultures (rectal swabs) at two university hospitals. Resistance identified by PCR sequencing. Conjugation experiments, PCR-based replicon typing, PFGE multilocus sequence typing (MLST) performed. assays showed, the isolates, twenty-nine strains produced carbapenemase genes. predominant blaOXA-48 (82.8%), followed blaNDM-1 (31%), blaNDM-7 (6.9%) blaOXA-181 (3.4%). Seven blaNDM positive co-harbored carbapenemases. found IncA/C IncL/M conjugative plasmids, respectively. blaCTX-M-15, qnrA intI1 also present most isolates. revealed genetic diversity 28 which belonged six minor clusters 14 singletons. 26 distributed into 18 STs, dominant (ST648 ST167). We one blaNDM-1-positive ST131 harbor blaCTX-M-15 blaTEM Horizontal transfer plasmids has likely facilitated spread coli. Their clonal presence faecal carriers suggest an endemic OXA-48 NDM. Therefore, it emphasizes critical importance monitoring controlling
sci-hub.se 参考文章(38)
Wafaa Y. Jamal, M. John Albert, Fatima Khodakhast, Laurent Poirel, Vincent O. Rotimi, Emergence of New Sequence Type OXA-48 Carbapenemase-Producing Enterobacteriaceae in Kuwait Microbial Drug Resistance. ,vol. 21, pp. 329- ,(2014) , 10.1089/MDR.2014.0123
P. Nordmann, L. Poirel, The difficult-to-control spread of carbapenemase producers among Enterobacteriaceae worldwide Clinical Microbiology and Infection. ,vol. 20, pp. 821- 830 ,(2014) , 10.1111/1469-0691.12719
Yanbin Liu, Yu Feng, Wenjing Wu, Yi Xie, Xiaohui Wang, Xiaoxia Zhang, Xinchuan Chen, Zhiyong Zong, First Report of OXA-181-Producing Escherichia coli in China and Characterization of the Isolate Using Whole-Genome Sequencing Antimicrobial Agents and Chemotherapy. ,vol. 59, pp. 5022- 5025 ,(2015) , 10.1128/AAC.00442-15
M.N.D. Balm, M.-V. La, P. Krishnan, R. Jureen, R.T.P. Lin, J.W.P. Teo, Emergence of Klebsiella pneumoniae co-producing NDM-type and OXA-181 carbapenemases Clinical Microbiology and Infection. ,vol. 19, ,(2013) , 10.1111/1469-0691.12247
Pedro Torres-González, Miriam Bobadilla-del Valle, Estrella Tovar-Calderón, Francisco Leal-Vega, Araceli Hernández-Cruz, Areli Martínez-Gamboa, María Dolores Niembro-Ortega, José Sifuentes-Osornio, Alfredo Ponce-de-León, Outbreak Caused by Enterobacteriaceae Harboring NDM-1 Metallo-β-Lactamase Carried in an IncFII Plasmid in a Tertiary Care Hospital in Mexico City Antimicrobial Agents and Chemotherapy. ,vol. 59, pp. 7080- 7083 ,(2015) , 10.1128/AAC.00055-15
F C Tenover, R D Arbeit, R V Goering, P A Mickelsen, B E Murray, D H Persing, B Swaminathan, Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. Journal of clinical microbiology. ,vol. 33, pp. 2233- 2239 ,(1995) , 10.1128/JCM.33.9.2233-2239.1995
Sebastien Breurec, Alfred Kinana, Abdoulaye Seck, Benoit Garin, Olivier Moquet, Coralie Bouchiat, Raymond Bercion, Omar Arouna, Class D OXA-48 carbapenemase in multidrug-resistant enterobacteria, Senegal. Emerging Infectious Diseases. ,vol. 17, pp. 143- 144 ,(2011) , 10.3201/EID1701.100224
Gisele Peirano, Patricia A Bradford, Krystyna M Kazmierczak, Robert E Badal, Meredith Hackel, Daryl J Hoban, Johann DD Pitout, None, Global Incidence of Carbapenemase-Producing Escherichia coli ST131 Emerging Infectious Diseases. ,vol. 20, pp. 1928- 1931 ,(2014) , 10.3201/EID2011.141388
Laurent Poirel, Laurent Dortet, Sandrine Bernabeu, Patrice Nordmann, Genetic Features of blaNDM-1-Positive Enterobacteriaceae Antimicrobial Agents and Chemotherapy. ,vol. 55, pp. 5403- 5407 ,(2011) , 10.1128/AAC.00585-11
Laurent Poirel, Timothy R. Walsh, Vincent Cuvillier, Patrice Nordmann, Multiplex PCR for detection of acquired carbapenemase genes Diagnostic Microbiology and Infectious Disease. ,vol. 70, pp. 119- 123 ,(2011) , 10.1016/J.DIAGMICROBIO.2010.12.002