Association of glutathione S-transferase (GSTM1, T1 and P1) gene polymorphisms with type 2 diabetes mellitus in north Indian population

摘要: Background: Diabetes mellitus is associated with an increased production of reactive oxygen species (ROS) and a reduction in antioxidant defense. The oxidative stress becomes evident as result accumulation ROS conditions inflammation Type 2 diabetes (T2DM). genes involved redox balance, which determines the susceptibility to T2DM remain unclear. In humans, glutathione S-transferase (GST) family comprises several classes GST isozymes, polymorphic variants GSTM1, T1 P1 decreased or loss enzyme activity. Aims: present study evaluated effect genetic polymorphisms gene on risk developing North Indian population. Settings Design: were genotyped 100 patients 200 healthy controls from India analyze their association susceptibility. Materials Methods: Analysis GSTM1 GSTT1 was performed by multiplex polymerase chain reaction (PCR) GSTP1 PCR-Restriction Fragment Length Polymorphism (RFLP). Statistical Analysis: Fisher's exact test χ2 statistics using SPSS software (Version-15.0). Results: We observed significant null (P=0.004, OR= 2.042, 95%CI= 1.254-3.325) (I/V) (P=0.001, 0.397, 95%CI=0.225-0.701) no (P=0.493). combined analysis three genotypes null, (I/I) demonstrated increase (P= 0.005, 2.431 95% CI=1.315-4.496). Conclusions: This first showing representative cohort T2DM. Since seen multiple (I/I), these can be screened population determine diabetic risk.