Osteopontin Reduces Polyspermy During In Vitro Fertilization of Porcine Oocytes

摘要: This study was designed to determine the role of osteopontin (SPP1) in vitro fertilization (IVF) swine. The initial objective evaluate effect various concentrations SPP1 (0, 0.001, 0.01, 0.1 and 1 μg/ml) on spermatozoa oocytes during IVF. results demonstrate that reduced rate polyspermy a dose-dependent manner (P < 0.05). also both number sperm as compared control bound zona pellucida (ZP) High doses (1 penetration male pronucleus formation Interestingly, group, medium increased efficiency (42.6% 44.6% vs. 31.6%; P 0.05), representing 41% improvement for μg/ml SPP1). ZP SPP1-treated more difficult digest than percentage acrosome-reacted spermato zoa IVF after 4 h 1.0 treatment 0 or SPP1. did not have an motility, progressive viability. To confirm reduction specific SPP1, mixture pregnancy-associated glycoproteins included protocol shown no polyspermy. Furthermore, Western blotting demonstrated 50-kDa form present oviducts Days 0, 3, 5 pregnant nonpregnant gilts, concentration Day higher 3 5. current represents first report plays important regulation pig polyspermic fertilization; it decreases increases