摘要: In recent years, progress has been achieved in the production of pig embryos through IVM and IVF techniques. Cytoplasmic maturation oocytes improved by modifications to procedures. However, historical problem polyspermic penetration still remains a major issue be solved. Recent studies indicate that type medium certain can reduce polyspermy. Efforts should directed increase developmental competence quality embryos. At present, many embryo culture (EC) media are available overcome 4-cell block support development early vivo derived blastocyst stage. contrast, vitro produced these varies significantly. Furthermore, morphology cell numbers blastocysts inferior their counterparts. several EC techniques have competence. Testing viability surgical transfer recipient animals resulted acceptable pregnancy rates with moderate litter sizes. Although reliable systems for generation embryos, polyspermy poor hamper large-scale implementation. Further research efforts improve oocyte/embryo methods minimize novel IVM, IVF,